Metabolism process
Metabolic processes are the basic activities in all lives and are
very sensitive to environmental changes. In present study, 12-
oxophytodienoate reductase (spot 17) involved in jasmonate
biosynthesis was induced by salt stress. Jasmonate is a signaling
compound which influences multiple cellular functions, implying
salt stress stimulated jasmonate signal transduction network.
Biotin carboxylase (spot 18) catalyzes the ATP-dependent carboxylation
of acetyl-CoA to produce malonyl-CoA, and this reaction is
the first committed step of de novo fatty acid biosynthesis [15].
Fatty acids are crucial components of cellular membranes,
contributing to inducible stress resistance through the remodeling
of membrane fluidity. Carbonic anhydrase (spot 26 and 27), which
catalyzes the reversible inter-conversion of HCO3
and CO2 involved
in pH regulation [16], was induced by salt stress and might regulate
cell pH to resisted damage by salinity. Glycine dehydrogenase (spot
4) participating in glycine, serine and threonine metabolisms was
up-regulated, and ribonuclease UK114 (spot 48) involved in nucleic
acid degradation was down-regulated, indicating that amino acid
and nucleic acid metabolisms were also affected by salt stress. In
addition, salt stress has a negative effect on the level of five spots
including thiazole biosynthetic enzyme (THI1, spots 11, 12),