2.5. Production and Purification of Recombinant rESAT6 and rCFP10.
The recombinant rESAT6 and rCFP10 (fused with GST peptide), used in the experiments as a control, were produced beforehand in E. coli strain BL21 (DE3). The recombinant proteins were affinity purified on Glutathione Sepharose 4B (Pharmacia, Sweden) as earlier described [24]