Trichodermaspp. isolated from rhizosphere of banana (Musasp.) from different areas of Tamil Nadu, India were evaluated
under in vitro condition for their antagonistic potential againstFusarium oxysporum, the banana fusarium wilt pathogen.
Trichoderma harzianumisolate Th-10 was the most effective in inhibiting the mycelial growth of fusarium in vitro. Out of
five different organic substrates (rice bran, rice chaffy grain, farmyard manure, banana pseudostem and dried banana leaf)
tested, dried banana leaf was the best carrier material to supportT. harzianumgrowth. Strain Th-10 colonized the dried banana
leaves within few days and produced high density of propagules (4.6×10
32
cfu/g of leaf). Addition of jaggery (10% w/v) to
the dried banana leaves increased the multiplication of T. harzianumwhich survived for >6 months on the stored substrate.
When applied as dried formulation, the population ofT.harzianumTh-10 increased from 10
4
to 10
13
cfu/g of soil within 60
days. In two field trials, soil application ofT.harzianumTh-10 as dried formulation effectively controlled fusarium wilt with
an efficacy comparable to that of the fungicide carbendazim.
© 2003 Elsevier B.V. All rights reserved.
Trichodermaspp. isolated from rhizosphere of banana (Musasp.) from different areas of Tamil Nadu, India were evaluatedunder in vitro condition for their antagonistic potential againstFusarium oxysporum, the banana fusarium wilt pathogen.Trichoderma harzianumisolate Th-10 was the most effective in inhibiting the mycelial growth of fusarium in vitro. Out offive different organic substrates (rice bran, rice chaffy grain, farmyard manure, banana pseudostem and dried banana leaf)tested, dried banana leaf was the best carrier material to supportT. harzianumgrowth. Strain Th-10 colonized the dried bananaleaves within few days and produced high density of propagules (4.6×1032cfu/g of leaf). Addition of jaggery (10% w/v) tothe dried banana leaves increased the multiplication of T. harzianumwhich survived for >6 months on the stored substrate.When applied as dried formulation, the population ofT.harzianumTh-10 increased from 104to 1013cfu/g of soil within 60days. In two field trials, soil application ofT.harzianumTh-10 as dried formulation effectively controlled fusarium wilt withan efficacy comparable to that of the fungicide carbendazim.© 2003 Elsevier B.V. All rights reserved.
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