Vitamins are essential for sporulation. Previous experiments performed using the sporulating fungi Coniothyrium minitans indicate that thiamine is the most critical vitamin for sporulation. Without this vitamin no sporulationoccurs [17]. In this study a mixture of several vitamins was tested and the results were compared with the results obtained using only thiamine (Figure 2(a)). It was observed that the spore yield obtained using 10 mg∙L−1 of thiamine was similar to the result obtained using the vitamin cocktail, which is advantageous since it is less expensive and it is easier to prepare.