2.3. Extraction
Ripe pineapples were cut into pieces and washed with deionised
water. The small pieces were homogenised in a grinder for 3 min to
40-mesh size paste. Twenty five grams of paste samples was extracted
with 150 mL of ethyl acetate by mixing, using a magnetic stirrer at
30 °C for 2 h. The extract was filtered through Whatman No. 41 filter
paper to obtain particle free extract. The residue was reextracted
twice and filtered. The extracts were pooled and concentrated and
dried under vacuum. The same procedure was followed for the
other solvents such as methanol and water for antioxidant fractions
(Jena et al., 2002) and the extracts were used to explore their antioxidant
activity.