Samples (2 mL) were loaded on a
Biogel P4 column (100 cm 3 cm) coupled to a Biogel P2 column
(100 cm 3 cm). Both columns were kept at 60 C and eluted with
water at a flow rate of 0.8 mL/min. Compounds with a molecular
weight (MW) between approximately 500 and 2000 were
collected. This final fraction, containing oligosaccharides with a
degree of polymerization (DP) between 3 and 12, was lyophilized.