In the present study, Nitrosomonas loads in Pond A, B and control ponds ranged from 2.01×103 to 4.80×103 cfu/ml, 2.25×103 to 4.9×103 cfu/ml and 1.24×103 to 2.00×103 cfu/ml, and Nitrobacter loads from 2.20×103 to 4.95×103, 2.10×103 to 4.15×103 and 1.10×103 to 2.15×103, respectively