The TEM image of typical nano zinc oxide is shown in Fig. 1A. It can be seen that the synthesized nano zinc oxide particles are in different shape and agglomerate possessing an average diameter of 25 ± 5 nm. At higher resolution, the inter-planar d-spacing of the lattice plane 0.246 nm is clearly visible in Fig. 1A2, corresponding to the (1 0 1) planes of wurtzite zinc oxide which is in agreement with previously reported values (Zhao et al., 2014). These studies clearly specify that the precipitation route supports the formation of the well-defined structure of zinc oxide material, which enhances their applicability in medical and advanced material science applications.