According to growth obtained in PDA medium, of −4.19 MPa and −8.52 MPa were chosen tomodify MLA medium.
The awof the media was determinedusing an equipment AquaLab (Series 4, TE, USA).
The mediawere autoclaved at 121◦C for 20 min before cooling to 50◦Cand pouring into 9 cm sterile plastic Petri plates.