The distinct factions were recovered in ddH2O and tested for antimicrobial activity against R. solanacearum. The fraction that exhibited strong antimicrobial activity in vitro was further analyzed by an HPLC system (Agilent 1200). The mobile phase was a 45% CH2CN solution, containing 0.1% H3PO4, with a speed of 1.0 ml/min. The detection's wavelength was fixed at 270 nm.