These tissue sections were placed on glass slides fixed in methanol for 30 s, and then washed gently with distilled water, followed by staining with toluidine blue (0.1 g/L) for 5e10 s, washed again and air dried.
Thesetissue sections were placed on glass slides fixed in methanol for30 s, and then washed gently with distilled water, followed bystaining with toluidine blue (0.1 g/L) for 5e10 s, washed again andair dried.