The digesters were five gallon capacity. A hole was bored on the top of the cap of the
digester; tube from a urine bag was inserted through the hole and glued using araldite to
make it air tight. The slurry was prepared from the stored sample by taking 400g in separate
beaker and 2000 cm3 of water was added to the substrate to obtain a ratio 1:5 W/V, mixed
thoroughly and transferred to the digester