2.6. Antibacterial Experiments in LB Plates. A 0.05 mL aliquot
of an E. coli suspension was mixed with 0.05 mL of 2.00 mg/mL
HSSNs, pure H2O, or polymyxin B-doped HSSNs with a
concentration of 1.00, 0.50, 0.25, and 0.10 mg/mL, respectively.
Each as-prepared 0.10 mL bacteria−sample mixture was evenly spread
on the surface of LB-agar dishes. The dishes were then placed in an
incubator for 24 h at 37 °C