Two facultative cultures, E. coli and Pseudomonas
sp., were obtained from the Microbiology Laboratory of
the Medicine Faculty at Aegean University and inoculated
into nutrient broth (1 g/l beef extract, 2 g/l yeast
extract, and 5 g/l NaCl) medium. These bacteria were
facultative anaerobes capable of growing under both
aerobic and anaerobic conditions.
These bacteria were cultivated in nutrient broth
medium under aerobic, anaerobic and microaerophilic
conditions. The anaerobic incubation was carried out in
3.5 l glass anaerobic bottles, AnaerobGen (OXOID) at