Sample extraction
Extraction efficiencies of different solvents, namely, dichloro-
methane, ethyl acetate, methanol, and aqueous methanol (50%)were tested on C. longa and E. elatior. Leaves of the two species
(1 g) were powdered with liquid nitrogen in a mortar and ex-
tracted with 50 ml of solvent, with continuous swirling for one
hour at room temperature using an orbital shaker. Extracts were
filtered under suction and stored at ?20 ?C for further use. Analysis
of extracts was done in triplicate.
For subsequent analyses, fresh and dried leaves of A. zerumbet,
E. elatior, C. longa, and K. galanga were extracted with methanol.
For the analysis of AOP of tea extracts, 1 g of tea in powder form
was extracted in 50 ml boiling water for 1 h with continuous swirl-
ing. The infusions were allowed to cool throughout extraction per-
iod. Extracts were filtered and stored at 4 ?C for further analysis.
Sample extractionExtraction efficiencies of different solvents, namely, dichloro-methane, ethyl acetate, methanol, and aqueous methanol (50%)were tested on C. longa and E. elatior. Leaves of the two species(1 g) were powdered with liquid nitrogen in a mortar and ex-tracted with 50 ml of solvent, with continuous swirling for onehour at room temperature using an orbital shaker. Extracts werefiltered under suction and stored at ?20 ?C for further use. Analysisof extracts was done in triplicate.For subsequent analyses, fresh and dried leaves of A. zerumbet,E. elatior, C. longa, and K. galanga were extracted with methanol.For the analysis of AOP of tea extracts, 1 g of tea in powder formwas extracted in 50 ml boiling water for 1 h with continuous swirl-ing. The infusions were allowed to cool throughout extraction per-iod. Extracts were filtered and stored at 4 ?C for further analysis.
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