Ten to fourteen days after plant flowering, stripped out of the seeds, disinfected the surface, got rid of the immature embryo and took the scutella as the test material. Osmotic treatment of immature embryo scutella was done for 4 h with 0.4 M mannitol after it two days preculture. Bombardments were carried out with particle bombardment (PDS 1000/He, Bio-Rad) under a vacuum of 27 inches of Hg and the conversion process refers to improvement methods of Rasco et al. (1999).