For examining antagonism due to diffusible com-pounds, a fungal lawn of the test fungus was grownon potato dextrose agar plates. Discs of 7 mmdiameter from the fungal lawns were cut andinoculated on carrot potato agar plates. A sterilisedpaper disc with 5 mm diameter, dipped in yeastextract broth containing the bacterial culture(108cfu ml1) was inoculated about 2.0–2.5 cm awayfrom the fungal disc. The plates were incubated ininverted position at 281Cinthedark.Theobserva-tions were recorded after 24, 72, and 120 h incuba-tion by measuring the growth of the fungus towardsand away from the bacterial colony. Inhibition offungal growth was calculated using the formula: