2.8. DSC analysis of the skin
Thermotropic properties of the skin after in vivo permeation study were analyzed using differential scanning calorimeter (TGA/DSC, 1/1600HT, METTLER, Switzerland). Briefly, the treated skins after in vivo permeation study were removed and rinsed with distilled water. Then the skin was blotted with tissue paper and fragmented, respectively. The small pieces were kept in a desiccator for 48h to dehydrate (Kawadkar et al., 2013). In DSC studies, samples were weighed precisely and placed into aluminum crimp cells and heated at a rate of 10C/min with a heating range of 30–400 C.