Based on central composite rotatable experimental design and response surface method, the interacting
effects of temperature (20 Ce34 C) and dietary protein level (25%e50%) on hematological parameters
including red blood cell (RBC), white blood cell (WBC) and hemoglobin (Hb) of juvenile Oreochromis
niloticus were studied under laboratory conditions. The experiment lasted for 7 weeks. After the feeding
trial, fish were challenged with Streptococcus iniae and mortality was recorded for within 8 days. Results
showed that the linear and quadratic effects of temperature on RBC, WBC and Hb were highly significant
(P < 0.01). When the dietary protein level was 25%e50%, the RBC, WBC and Hb were increased firstly and
then decreased, but the linear and quadratic effects of protein level were insignificant
Based on central composite rotatable experimental design and response surface method, the interactingeffects of temperature (20 Ce34 C) and dietary protein level (25%e50%) on hematological parametersincluding red blood cell (RBC), white blood cell (WBC) and hemoglobin (Hb) of juvenile Oreochromisniloticus were studied under laboratory conditions. The experiment lasted for 7 weeks. After the feedingtrial, fish were challenged with Streptococcus iniae and mortality was recorded for within 8 days. Resultsshowed that the linear and quadratic effects of temperature on RBC, WBC and Hb were highly significant(P < 0.01). When the dietary protein level was 25%e50%, the RBC, WBC and Hb were increased firstly andthen decreased, but the linear and quadratic effects of protein level were insignificant
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