Taken together, shrimps cultured in inland ponds can be reservoirs of a variety of oxytetracycline-resistance genes, originating from both inland and marine environments, although details of the genes need to be further studied. Our results highlight that attention should be focused on the antimicrobial resistance of bacteria originating from both inland and marine environments in contemporary shrimp culture facilities. Furthermore, the shift of shrimp farming to inland environments might increase the opportunity for dissemination of resistance genes among a variety of bacteria from both inland and marine environments.