on the extraction yield, the oil solubility and the initial extraction rate were investigated. A cross-over effect for the extraction of pumpkin seed oil using supercritical CO2 was determined at the operating pressure of 20–30 MPa. The maximum extraction yield obtained with entrainer free was reached 0.50 g oil/g dry seed at 600–1180 μm, 0.12 L/h, 50 MPa and 70 °C for the operation time of 5 h.