Overnight cultures were used to inoculate 50 ml sealed bottles
containing 5 ml of E2 medium supplemented with different
hydrocarbons (crude oil, kerosene, diesel, octane, phenol, benzene,
toluene and xylene) to an initial DO600 of 0.05. Cultures were
incubated at 30 C and 300 rpm. Tolerance assays were performed
in LB medium supplemented with different concentrations of pure
hydrocarbons (Table 2). Growth and tolerance were analyzed by the
appearance of turbidity after 7 days of cultivation.