2.2.2. Physical methods
The pH was determined using Testo pH meter, type 230, Lenzkirch Germany, at room temperature (22 ± 3 °C) after mixing 10 g of homogenized sample in 100 ml of distilled water as described in AOAC method (AOAC, 2000). The loss in weight of frozen sample after thawing was calculated as percentage and reported as a thawing loss. The count per pound of the shrimps in each sample was calculated and its range was recorded.
2.2.3. Statistical analysis
Mean, range and standard deviation were calculated as described by Frank and Althoen (1994).