Conclusions The present study emphasis potato pulp powder as a new organic substrate for the production of acid protease. The improved A. oryzae strain (F6) on SSF with potato pulp powder and statistically optimized bioprocess variables namely50% moisture, 30◦C, 120 h fermentation time enabled 31 U/gdry substrate acid protease production. The enzyme alsoreleased 0.57 ± 0.25 U/g of glycine which is beneficial and offer wide applications as well as improved prospects in the foodindustry. The results demonstrate an economically viable acidprotease enzyme production process using a byproduct of the potato processing industry.