Bacterial strains utilized in this study are described in
Table 1. For general growth, strains were grown overnight in
Luria-Bertani (LB) broth at 37 8C with shaking at 200 rpm. Tryptic
soy broth diluted in phosphate buffered saline (TSB:PBS) at 1:25 for
P. aeruginosa and 1:10 for S. aureus was used for all other
experiments described in this study. To examine the biofilms using
confocal laser scanning microscopy, we utilized the P. aeruginosa
strain PAO1/pMP7605 and the S. aureus strain AH133. Strain PAO1/
pMP7605 carries plasmid pMP7605 in which the gene that code