FIG. 3. Temperature dependence of the phosphomolybdenum assay
for different reducing species. Identical concentrations (5 mM) of
four different reducing species were assayed at 37°C (A) or 95°C (B).
The formation of the phosphomolybdenum complex was monitored at
the times indicated by measuring the absorbance at 695 nm. a-Tocopherol
(F), ascorbic acid (E), reduced glutathione (), BHT (ƒ). All
the points represent the average of three different measurements,
and deviations were always below 1%.