The transport sector is the dominant source of nanoparticles in the urban atmosphere. It is also responsible for about 20–25% of current global CO2 emissions, a figure that is expected to grow to about 30–50% by 2050 (Fuglestvedt et al., 2008). One option to counter this trend and contribute to the attainment of carbon emission reduction targets is the use of biofuels in road vehicles. This leads to a reduction in CO, CO2 and particle mass emissions, though particle number emissions may increase. This article discusses the potential impact of the particle number concentrations derived from biofuel vehicles on existing regulatory concerns over atmospheric nanoparticles. Copyright © 2010 Royal Meteorological Society