Fifty microliters of hemolymph were withdrawn from
the ventral sinus of each shrimp from five shrimps per tank
into a syringe and transferred to Eppendorf tube containing
450 ìl trypan blue solution. A drop of haemolymph in trypan
blue solution was placed on hemocytometer and a total
haemocyte count (THC) was determined using a stereomicroscope
(