Tween 20 agar plates were prepared as follows; the culture
medium contained peptone 1 % (w/v), NaCl (0.5 % w/v),
CaCl2 (0.01 % w/v), agar (2 % w/v) and tween-20 (1 % v/v)
and the mixture was adjusted at pH 6.0 using 1 M NaCl.
About 20 ml of the medium was poured into each petri dish
and the organism inoculated. The appearance of visible clear
zone was used as an indicated of lypolitic activity.