The neutral detergent fiber (NDF using
heat-resistant a-amylase without sodium sulfite), acid detergent
fiber (ADF) and acid detergent lignin (ADL) were analyzed
according to Mertens (2002), AOAC (1990) (973.187),
and Van Soest et al. (1991), respectively, using the sequential
procedure and the filter bag system (Ankom Technology,
New York). The NDF and ADF fractions include residual
ash. N-free extract was calculated by difference. Hemicellulose
and cellulose were also estimated as NDF (ash free)  ADF
(ash free) and ADF (ash free) – ADL (ash free), respectively
 
The neutral detergent fiber (NDF usingheat-resistant a-amylase without sodium sulfite), acid detergentfiber (ADF) and acid detergent lignin (ADL) were analyzedaccording to Mertens (2002), AOAC (1990) (973.187),and Van Soest et al. (1991), respectively, using the sequentialprocedure and the filter bag system (Ankom Technology,New York). The NDF and ADF fractions include residualash. N-free extract was calculated by difference. Hemicelluloseand cellulose were also estimated as NDF (ash free)  ADF(ash free) and ADF (ash free) – ADL (ash free), respectively
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