The CAT activity was measured by recording
H2O2 disappearance characterized by an absorbance
decrease at 240 nm according to a method described by
Aebi (1984), with modifications. A 5 g sample was mixed
with 25 mL of 50 mM phosphate buffer (pH 7.0) at
13,500 rpm using a homogenizer (Ultra-Turrax T25 basic,
IkaWerke GmbH & Co., Staufen im Breisgau, Germany) for