During a 2-wk depletion period, all pigs (initial BW:31.1 ± 4.2 kg) were housed individually in 0.9 by 1.8 m pens that had fully slatted concrete floors, a stainless steel feeder, and a stainless steel bowl-type drinker. All pigs were fed the corn grits diet containing BMM during this period. Pigs were then transferred to metabolism cages and randomly allotted to the 6 experimental diets. The average weight of the pigs at the time of transfer was 40.2 ± 6.2 kg. The stainless steel metabolism cages were equipped with a fully perforated floor, a screen floor for fecal collection, and a stainless steel tray for urine collection. The quantity of feed provided per pig daily was calculated as 3 times the estimated requirement for maintenance energy (i.e., 106 kcal ME per kg0.75; NRC, 1998) and divided into 2 equal meals. Water was provided by a
bowl-type drinker and available at all times. Experimental diets were provided for 12 d. The initial 5 d was considered
an adaptation period to the diet. Fecal markers were fed on d 6 and on d 11 and fecal collections were initiated
when the first marker appeared in the feces and ceased when the second marker appeared (Adeola, 2001). Urine
was collected from d 6 to 11 in urine buckets over a preservative of 50 mL of 6 N HCl. Buckets were covered
by gauze to prevent solids from contaminating the urine. Fecal samples and 20% of the collected urine were stored
at –20°C immediately after collection.