The HPLC system for neotame was the same as in the case of
aspartame. The mobile phase was 0.09% TFA, water: acetonitrile
(60:40) pumped at a flow rate of 0.6 ml/min. The column temperature
was maintained at 40 C in a column heater chamber. The
sample volume was 20 ll. A PDA detector at 210 nm was used to
monitor the eluate