The present work is first comprehensive report on the AFM 1
occurrence in raw milk produced in Macedonia during the 2013
incidence. Furthermore, it presents the results from the coordi-
nated milk-feed sampling with aim of detecting, and possibly
eliminating the contamination source. Regarding the samples
number, the first choice of method implemented for AFM 1 deter-
mining in milk was enzyme-linked immunoassay (ELISA). After the
initial screening, the suspicious positive results were analyzed with
high-performance liquid chromatography with fluorescence
detection (HPLC-FD). Bearing in mind the experiences of the pre-
viously conducted similar surveys, such a combination is likely to
be the most accurate scheme for high throughput testing
(Shephard, 2009).
The present work is first comprehensive report on the AFM 1occurrence in raw milk produced in Macedonia during the 2013incidence. Furthermore, it presents the results from the coordi-nated milk-feed sampling with aim of detecting, and possiblyeliminating the contamination source. Regarding the samplesnumber, the first choice of method implemented for AFM 1 deter-mining in milk was enzyme-linked immunoassay (ELISA). After theinitial screening, the suspicious positive results were analyzed withhigh-performance liquid chromatography with fluorescencedetection (HPLC-FD). Bearing in mind the experiences of the pre-viously conducted similar surveys, such a combination is likely tobe the most accurate scheme for high throughput testing(Shephard, 2009).
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