SEASON
The season of the year can fave effects on contamination and response in culture. For example , buds or shoots taken during the spring of the year when the shoots are in a flush state of growth are more responsive than dorment buds. As the season of the year passes from spring, summer, and fall to winter the explant generally does not respond as well in culture. Tissue that is physiologically dormant is generally unresponsive in culture until the dormancy requirement is met; sometimes the dormancy response can be met in culture such as chilling bulbscale explants or embryos to break dormancy. Additionnally, contamination rates also increase as the summer progresses; fall and winter contamination can increase to 100%.