Rumen fermentation and blood metabolites
As shown in Table 3, rumen temperature and pH were similar among treatments and values were quite stable. Buffaloes fed with UTRS had higher concentration of ruminal NH3-N and BUN as compared to RS. However, EuO supplementation did not affect on ruminal NH3-N and BUN concentrate. There were no differences in total volatile fatty acid (TVFA) and butyrate (C4) concentration among treatments by roughage source or EuO supplementation. Roughage sources and EuO supplementation influenced acetate (C2) and propionate (C3) concentrations and CH4 production. The treatments with UTRS resulted in lower C2, but higher C3 as compared to RS. EuO supplementation reduced C2 and increased C3 concentration in both RS and UTRS. Production of CH4 was reduced by EuO supplementation especially in buffaloes fed UTRS.