e) Add the volumes calculated in (a) above directly to the larval tank. The remainder (15.04 minus 1.67 l for Pavlova, etc.) is mixed in a cooled, aerated reservoir of sufficient volume. Dose this volume at a constant rate over the 24-hour period. From the practical point of view, it is advisable to make up the algae contained in the reservoir with filtered seawater to the volume pumped in 24 hours by the peristaltic pump.