Huperzia selago sporophyte in vitro cultures were initiated
using vegetative propagules (bulbils; Fig. 1, Fig. 2) procured
from different local populations in the Babia Góra National
Park (The Carpathians, Poland). They were harvested from
the mother sporophytes and protected from drying during
transport by placing on blotting paper moistened with water in
tightly closed containers. The plants were collected in spring,
from April to June, and in autumn, in September and October.
In the laboratory, the plants were cleaned by manually removing
litter, soil and leaves of other plants, and then rinsed in
a stream of running tap water for 1 hour. Voucher specimens,
including sporophytes with bulbils, were taken and deposited
in the herbarium at Department of Biology and Pharmaceutical
Botany, Medical University of Warsaw.