The PCR amplifications were carried out in a PTC-100 thermocycler (MJ Research Inc., St. Bruno, Canada) under the following conditions: an initial melting step at 94 °C for 5 min; followed by 35 cycles at 94 °C for 30 s, annealing temperatures as indicated in Table 2 for 60 s, and 72 °C for 30 s; and a final extension at 72 °C for 10 min.