leaf extract was evaluated based on DPPH, ABTS, NO and H2O2 radical scavenging activities and total phenolic assay. The result of the present study revealed that the leaf extract exhibited best antioxidant activity. It was observed that the leaf extract contained high level of phenolic content that might have accounted for the strong activity observed against ABTS and DPPH radicals. Despite the widespread use of A. malaccensis as folk medicines, literature contains few reports on its antioxidant activity. In the present experiment, we conceded a systematic record on the relative free radical scavenging activity in methanolic leaf extract. The finding of this study suggests that A. malaccensis leaves could be a potential source of natural antioxidant that could have great importance as therapeutic agents in preventing or slowing the progress of ageing and age associated oxidative stress related degenerative diseases. Further investigation on the isolation and characterization of the antioxidant constituents is however required.
ACKNOWLEDGEMENTS
The authors acknowledge DST-INSPIRE division, New Delhi for providing financial support. The authors are grateful to the Dean, Centre of Biotecnology, Siksha O Anusandhan University for providing the facilities to carry out this research work.