Banana (Musa sp.) is considered as one of the most important fruit crops worldwide
as well as in Egypt. The main goal of this study was to construct the open reading frame (ORF)
of banana bunchy top nanovirus (BBTV)-DNA-3 that encodes the viral coat protein (cp) gene
for banana transformation. The previously sequenced BBTV-G-DNA-3-ORF that cloned into
plasmid pH1 was used as a template for PCR amplification using two specific primers containing
Bam H1 site. A new plasmid called pRHA1 containing the amplified ORF under the control
of maize polyubiquitin (ubi) promoter was created. The bar gene (herbicide-resistance gene as a