FT-IR was employed for detection of changes in the functional
groups in the chemical structure of PCL film after incubation with
strain MRL-TL. 10 mg of PCL film was dissolved in dichloromethane
in a small test tube and then transferred with a pipette to IR plates
(PerkinElmer FTIR Spectroscopy, USA), a spectrum was taken in
single at 500e4000 wave-numbers cm1 of the sample. FTIR
spectrum of PCL film from abiotic control was taken by same procedure
and compared the results with test sample. Spectrum of
dichloromethane was also obtained and considered as baseline for
all the samples.