However,
direct examination of PCR-amplified clones in a given
sample is essential in the first stage of molecular
ecology study because minor components of microbiota
cannot be accurately identified by the highthroughput
methods mentioned. A total of 191 16S
rRNA gene sequences and 157 ARDRA patterns were
obtained from the five kimchi samples. 16S rRNA
gene sequences that were N98% similar to the type
strains were regarded as belonging to the corresponding
species or phylotypes. In the case of ARDRA,
identical restriction patterns to the type strains led to
assignment to the corresponding species.