Hot water treatment can control is of anthracnose (C. gloeosporioides) and stem end rot-causing (L. theobromae) pathogens. Using a sterile scalpel, five 1-cm2 of surface tissue from each diseasesymptom were excised from the fruit. Three fruit were used for each disease symptom. The tissue pieces were surface sterilized with chlorox solution for 30 s, rinsed twice in sterilewater for 1 min and blotted dry on sterile filter paper. Five tissue pieces per plate were placed on fresh potato dextrose agar (PDA) plates and incubated for 3-7 days at 25 °C. Fungal colonies clearly developing from the fruit tissues were isolated and identified by cultural and morphological methods. The pathogenicity of C. gloeosporioides and L. theobromae was established on naturally infected green matured mangoes.