Upon arrival at the laboratory,
semen was placed into a water bath at 37 ◦C and.
A subsample was removed for analysis and the remainder
was extended to a concentration of 75
×
106 sperm/mL
using a commercially available 5-day extender (Androhep;
Minitube of America, Verona, WI); and stored in a semen
cooling unit (Minitube of America, Verona, WI) at 17–18 ◦C.
Percentages of motile and progressively motile sperm; percentages
of morphologically normal and abnormal sperm;
concentrations of malondialdehyde (MDA); and the fatty
acid composition extracted from a subsample of spermatozoa
were evaluated prior to extension and on days 1 and 6
post-collection.