Then 4 mL of water was added to the interphase together with an equal volume of water-saturated chloroform and isoamyl alcohol (24/1, v/v); the mixture was homogenized, incubated on ice for 10 min, and then centrifuged at 10 000 ×g for 10 min at 4°C.
Then 4 mL of waterwas added to the interphase together with an equal volumeof water-saturated chloroform and isoamyl alcohol(24/1, v/v); the mixture was homogenized, incubatedon ice for 10 min, and then centrifuged at 10 000 ×gfor 10 min at 4°C.