Experiments were performed in a batch mode. SMFCs were
allowed to acclimatize for first three feed cycles of operation, and
afterwards the performance was monitored for 25 days covering
five feed cycles. Feeding was done at an interval of 5 days.
The COD (170–185 mg L−1) and ammonium nitrogen concentration
(4–6 mg L−1) were maintained by adding sucrose and ammonium
chloride for each feed cycle. The pH of feed water was maintained
by using 10 mM phosphate buffer. Performance ofthese SMFCs was
evaluated at ambient temperature in the range from 29 to 30 ◦C.
Later, minimum day temperature decreased to 10 ◦C due to winter
season and one of the validation experiment (SMFC-1) was continued
to operate at this lower temperature condition to evaluate
effect of temperature on performance of SMFC.