3.2. Elution time-DAD matrices
Fig. 2A shows the overlapped experimental DAD chromatograms for
the components of a typical validation sample, and the corresponding
absorption spectra. TBZ, FBZ and CBZ co-elute with a strong injection
peak, and thus they were not determined with DAD. The first analyte whose band is separated from the injection peak in the DAD chromatogram is dicamba, followed by IMZQ, CBL, NFZ, NAP, methiocarb and FM,
with different overlapping degrees. Among these latter compounds, CBL
and NAP possess fluorescent properties, and were also evaluated by
fluorescence (see below).