Analysis of MCP
After the precipitate was filtered, and lyophilized to producea yellow powder, the polysaccharide was obtained, and the per-centage yield was 2.3%. The total carbohydrate content and proteincontent of MCP was 72.6 ± 1.2% and 8.9 ± 0.2%, respectively. Theuronic acid content in MCP determined according to Blumenkrantzand Asboe-Hansen’s method was 20.1 ± 0.4%. The molecular weightof polysaccharide was determined in a range of 85–100 kDa. To fur-ther investigate the composition of MCP, HPLC technique was usedin this study. Specifically, different monosaccharide standards wererun on HPLC and their retention time was recorded to identify themonosaccharide component of MCP. The monosaccharide compo-sition analysis revealed it contained arabinose, xylose, galactoseand rhamnose in a ratio of 1.00:1.12:4.07:1.79 according to theretention time and peak areas of HPLC