RNA extraction was done in a buffer containing 8 M guanidinium chloride, 20 HIM Mes, 20 mM EDTA, and 50 mM /3-mercaptoethanol, pH 7.0. RNA samples were separated on formaldehyde gel, transferred to the Zetaprobe membranes, and probed as above
RNA extraction was done in a buffercontaining 8 M guanidinium chloride, 20 HIM Mes, 20 mMEDTA, and 50 mM /3-mercaptoethanol, pH 7.0. RNA sampleswere separated on formaldehyde gel, transferred to the Zetaprobemembranes, and probed as above